L-Anserine (CAS 584-85-0) is supplied by TCS NEXUS S.L. (Valencia, Spain) for European cosmetic formulators, brand R&D teams and B2B buyers reviewing cosmetic peptide raw material applications. A freely water-soluble peptide, it dissolves directly in aqueous concentrates without co-solvents at pH 4.5–7.0, with cool-down addition below 40°C recommended in standard serum and leave-on manufacturing protocols.
> Technical Specifications
| Product Name | L-Anserine |
|---|---|
| Molecular Structure |  |
| CAS Number | 584-85-0 |
| Alias / Common Name | L-Anserine; β-alanyl-1-methylhistidine; N-β-alanyl-1-methyl-L-histidine |
| Molecular Formula | C10H16N4O3 |
| Molecular Weight | 240.3 g/mol |
| INCI Name | Anserine |
| Peptide Sequence | β-Ala-1-methylhistidine — β-alanyl-1-Me-His (1-methylhistidine imidazole dipeptide) |
| Functional Class | Naturally occurring imidazole dipeptide; antioxidant; carbonyl scavenger; anti-glycation active; histidine-containing dipeptide (HCD) class |
| Purity / Assay | ≥98% (HPLC); confirm by batch COA |
| Appearance | White to off-white crystalline powder |
| Solubility / Handling | Freely water-soluble at evaluation concentrations (0.1–2.0%); dissolves directly in deionised water or the aqueous phase without co-solvent. Add during the cool-down phase below 40°C. |
| pH Stability | Optimal stability at pH 4.5–7.0. Confirm stability in the complete formulation base. |
| Storage | Store sealed below 25°C in a cool, dry location protected from direct light, moisture and oxygen. Follow SDS and batch COA conditions. |
> Mechanism & Positioning
L-Anserine is a naturally occurring histidine-containing dipeptide (HCD) of the β-alanyl-imidazole class, structurally related to L-Carnosine but bearing a 1-methyl substitution on the histidine imidazole ring. The 1-methylhistidine residue confers resistance to carnosinase (CN1/CN2) enzymatic hydrolysis — a critical advantage for topical delivery since carnosinase activity in the epidermis limits peptide half-life for unsubstituted L-Carnosine. Published L-Anserine literature discusses carbonyl scavenging via nucleophilic imidazole ring addition to reactive carbonyl species (RCS): methylglyoxal (MGO), glyoxal (GO) and 4-hydroxynonenal (4-HNE) — key precursors of advanced glycation endproducts (AGEs) and advanced lipoxidation endproducts (ALEs) in skin. Cell-based fibroblast model studies document reduction in AGE accumulation (CML, GOLD crosslinks) and decreased carbonyl-modified collagen in dermal matrix model systems following L-Anserine treatment. Metal chelation model data also notes L-Anserine Fe²⁺ and Cu²⁺ chelation, supplementing the carbonyl-scavenging mechanism with transition metal-dependent oxidative stress attenuation.
L-Anserine (1-Me-His HCD) pairs with L-Balenine (3-Me-His HCD) for complementary methylhistidine isomer coverage, and with L-Carnosine (unsubstituted His) for the carnosinase-sensitive HCD component targeting intracellular environments. For comprehensive anti-AGE positioning, add Ergothioneine for TAUT-mediated intracellular antioxidant support and Acetyl Tetrapeptide-5 (Eyeseryl) for periorbital anti-glycation and anti-puffiness positioning. Contact info@tcspeptides.com with formulation specification.
> Application Concepts
Anti-Glycation Serum and Carnosinase-Resistant HCD Architecture
L-Anserine at 0.1–2.0% dissolves directly in deionised water and is added at cool-down below 40°C — a clean, straightforward addition to aqueous anti-age serums, regenerative essences and brightening concentrates without co-solvent or pre-dispersion. Its carnosinase resistance (1-methylhistidine blocks CN1/CN2 hydrolysis) makes it more appropriate than L-Carnosine for topical applications where formulation-to-epidermis transit time may expose the peptide to enzymatic degradation. For a premium three-HCD anti-glycation serum, combine L-Anserine (0.5–1.0%), L-Balenine (0.5–1.0%) and L-Carnosine (0.5–1.0%) in a common aqueous base at pH 5.5–6.5 for multi-modal HCD coverage.
Carnosinase Resistance — Why the 1-Methylhistidine Substitution Matters for Topical Stability
Carnosinase enzymes (CN1 — serum carnosinase; CN2 — cytosolic carnosinase) hydrolyse the β-alanyl-His amide bond of L-Carnosine in vivo. In skin, CN2 activity is present in keratinocytes and fibroblasts, meaning unprotected L-Carnosine applied topically is susceptible to enzymatic cleavage in the stratum granulosum and viable epidermis. The N1-methylhistidine substitution in L-Anserine and the N3-methylhistidine in L-Balenine both confer significant carnosinase resistance — these peptides retain the carbonyl-scavenging imidazole pharmacophore while resisting enzymatic degradation. For applications targeting the dermis (glycation of collagen fibres), carnosinase-resistant HCDs are more appropriate than unsubstituted L-Carnosine unless a lipid-conjugated delivery vehicle is employed. For intracellular antioxidant applications relying on CN2 hydrolysis to release free β-alanine and histidine, L-Carnosine retains an advantage.
Age-Care, Collagen Anti-Glycation and Long-Wear Brightening Format Applications
L-Anserine is positioned for three anti-ageing market segments where extracellular AGE inhibition is the primary claim basis: premium collagen-preservation age-care serums (dermal collagen glycation protection), daily anti-AGE moisturisers (prophylactic carbonyl scavenging), and brightening serums where glycation-driven yellowness or dullness is part of the ageing picture. L-Anserine at 0.1–2.0% integrates cleanly into fragrance-free, preservative-minimal bases targeting functional ageing claims. For brightening alignment, pair with Ergothioneine and niacinamide (4–5%) — antioxidant and melanosome-transfer inhibition alongside direct AGE carbonyl scavenging for a multi-mechanism anti-dullness serum strategy.
> Handling & Formulation Notes
Dissolve L-Anserine directly in deionised water or the aqueous phase at target concentrations (0.1–2.0%) without co-solvent or pre-dispersion step. Add to the formulation at cool-down below 40°C. Protect bulk powder from open-air exposure — store in nitrogen-blanketed or tightly sealed containers to minimise oxidation of the 1-methylhistidine imidazole ring. Reseal immediately after each use.
Formulate at pH 4.5–7.0; a finished serum pH of 5.5–6.5 is optimal for L-Anserine stability across the anti-age formulation pH range. Confirm stability of L-Anserine in the complete formulation at 40°C/75% RH for 12 weeks before scale-up. Monitor imidazole ring integrity by HPLC when formulating with strong oxidising agents or at pH outside 4.5–7.0. Follow SDS and batch COA for storage and handling; confirm shelf life for the specific batch.
> Supply & Documentation
L-Anserine (CAS 584-85-0) is available from TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic raw material projects. Standard documentation: COA, TDS, SDS. Sample and bulk quantities are discussed on a per-project basis; contact info@tcspeptides.com to initiate a sourcing review.
TCS NEXUS S.L. supports procurement and formulation teams in Germany, France, Italy, Spain, the United Kingdom, the Netherlands, Belgium, Poland, Sweden and Portugal, as well as international buyers in the United States, Canada, Japan, South Korea, Australia and New Zealand.
> Packaging & Storage
L-Anserine is supplied in sealed aluminium foil bags or bulk containers. Minimum quantities and packaging configurations are confirmed per order. Store below 25°C in a cool, dry location, away from moisture and direct light. Follow batch COA and SDS for specific storage and handling conditions.
> FAQ
What is L-Anserine?
L-Anserine (CAS 584-85-0) is a cosmetic raw material supplied by TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic formulators. In cosmetic literature, L-Anserine is positioned as a carnosinase-resistant dipeptide antioxidant and anti-glycation active — evaluated in anti-ageing and skin-clarity serums where its 1-methylhistidine imidazole ring confers protection against oxidative and carbonyl stress beyond that achievable with standard L-Carnosine. TCS NEXUS S.L. supplies L-Anserine (β-Ala-1-Me-His) as a B2B cosmetic active for anti-ageing formulation specialists.
What is the CAS number for L-Anserine?
The CAS number for L-Anserine is 584-85-0. The INCI name for cosmetic regulatory documentation and CPNP notification is Anserine; confirm CAS 584-85-0 and INCI identity against the batch COA when building product files.
How does L-Anserine act as an antioxidant — what does the 1-methyl group do?
L-Anserine (β-alanyl-1-methyl-L-histidine) is a naturally occurring histidine-containing dipeptide (HCD) closely related to carnosine, carrying a methyl group at position 1 of the imidazole ring. That methylation shifts the imidazole pKa and tunes its behaviour while keeping the core activity: scavenging reactive carbonyls and radicals and chelating metals for antioxidant and anti-glycation positioning.
What use level of L-Anserine is typical, and how should it be evaluated?
The appropriate dose is formulation- and claim-dependent, so build it up conservatively from your own evaluation instead of fixing on one published number. We supply pure L-Anserine with COA; contact info@tcspeptides.com to align documentation with your target.
Which actives pair with L-Anserine in an antioxidant HCD blend?
L-Anserine combines naturally with the other imidazole dipeptides — L-Carnosine and L-Balenine — and with antioxidant actives such as niacinamide and vitamin E for a multi-component HCD antioxidant system. It is water-soluble; confirm combined stability and pH in the finished base.
How does L-Anserine differ from L-Carnosine and L-Balenine?
The three are closely related histidine-containing dipeptides that differ in imidazole methylation: L-Carnosine is unmethylated (β-Ala-His), L-Anserine carries a 1-methyl group, and L-Balenine (ophidine) carries a 3-methyl group. The methyl position tunes pKa and antioxidant behaviour; formulators use them individually or as a naturally-inspired HCD blend. L-Anserine is freely water-soluble.
What addition temperature is recommended for L-Anserine during manufacturing?
Introduce L-Anserine during the cool-down phase at temperatures below 40°C; avoid prolonged exposure above this threshold. Standard aqueous or emulsion cool-down protocols are fully compatible with this addition requirement. Confirm thermal stability in the specific manufacturing process with accelerated stability data before committing to production scale.
What pH range keeps L-Anserine stable in a finished formula?
L-Anserine is stable across roughly pH 4.0–7.0; confirm the finished-product pH after all actives are incorporated and avoid strongly oxidising bases so the imidazole antioxidant activity is preserved.
Is L-Anserine compatible with other actives in multi-active cosmetic systems?
L-Anserine is compatible with standard cosmetic actives at pH 4.5–7.0 in aqueous or emulsion systems. Evaluate combined system pH drift, clarity and assay retention; confirm preservative compatibility. Run accelerated stability testing at 40°C/75% RH before scale-up.
Is Anserine (INCI) compliant with EU Cosmetics Regulation 1223/2009?
Anserine is not listed as a restricted, prohibited or regulated substance under EU Cosmetics Regulation 1223/2009 Annexes II–VI. The finished product manufacturer is responsible for the CPSR and CPNP notification. Use CAS 584-85-0 and INCI name Anserine consistently in all regulatory documentation; confirm purity and identity against the batch COA.
How should L-Anserine be stored?
Store L-Anserine sealed below 25°C in a cool, dry location protected from direct light and moisture. Once opened, reseal the container immediately. Follow specific storage and handling conditions stated in the SDS and batch COA. Confirm shelf life for the specific batch before incorporating into production formulations.
What documentation does TCS NEXUS S.L. provide for L-Anserine?
TCS NEXUS S.L. provides COA (confirming CAS 584-85-0 and HPLC purity ≥98% (HPLC)), TDS (Technical Data Sheet) and SDS (Safety Data Sheet) for L-Anserine. Additional documentation such as specification sheets and INCI confirmation letters is available on a per-project basis. Contact info@tcspeptides.com to initiate a documentation and sourcing discussion.
What MOQ and sample options are available for L-Anserine?
Sample and bulk quantities for L-Anserine are confirmed on a per-project basis through TCS NEXUS S.L. Minimum order quantities depend on current stock, packaging configuration and project scope. R&D teams are encouraged to request samples for stability and concentration-range evaluation before bulk planning. Contact info@tcspeptides.com with project specification to begin a sourcing review.
> Technical Support and Samples
Request COA documents, sample availability, or support for custom synthesis.