Biotinoyl Tripeptide-1 (CAS 299157-54-3) is supplied by TCS NEXUS S.L. (Valencia, Spain) for European cosmetic formulators, brand R&D teams and B2B buyers reviewing cosmetic peptide raw material applications. A freely water-soluble peptide, it dissolves directly in aqueous concentrates without co-solvents at pH 4.5–7.0, with cool-down addition below 40°C recommended in standard serum and leave-on manufacturing protocols.
> Technical Specifications
| Product Name | Biotinoyl Tripeptide-1 |
|---|---|
| Molecular Structure |  |
| CAS Number | 299157-54-3 |
| Alias / Common Name | Biotinoyl Tripeptide-1; Biotin-GHK; Biotinyl Tripeptide-1 |
| Molecular Formula | C24H38N8O6S |
| Molecular Weight | 566.7 g/mol |
| INCI Name | Biotinoyl Tripeptide-1 |
| Peptide Sequence | Biotin-Gly-His-Lys — Biotin-GHK (biotinylated GHK hair follicle targeting tripeptide) |
| Functional Class | Synthetic biotinylated tripeptide; hair follicle anchoring and proliferation active; fibronectin receptor modulator; lash, brow and scalp density peptide |
| Purity / Assay | ≥98% (HPLC); confirm by batch COA |
| Appearance | White to off-white powder |
| Solubility / Handling | Freely water-soluble at evaluation concentrations (0.001–0.1%); dissolves directly in deionised water or the aqueous phase without co-solvent. Add during the cool-down phase below 40°C. |
| pH Stability | Optimal stability at pH 4.5–7.0. Confirm stability in the complete formulation base. |
| Storage | Store sealed below 25°C in a cool, dry location protected from direct light and moisture. Follow SDS and batch COA conditions. |
> Mechanism & Positioning
Biotinoyl Tripeptide-1 (Biotin-GHK) is a synthetic biotinylated tripeptide combining the GHK copper-binding tripeptide backbone with a biotin (vitamin B7) moiety linked at the N-terminus. Published Biotinoyl Tripeptide-1 literature discusses dual-mechanism action at the hair follicle: the GHK sequence modulates fibronectin receptor signalling at the dermal papilla and hair matrix, promoting follicle anchoring to the dermal sheath, while the biotin moiety supports keratinocyte proliferation and keratin biosynthesis pathways in the hair matrix cortex. Cell-based dermal papilla cell studies document upregulation of fibronectin deposition, increased IGF-1 receptor signalling and improved follicle cell viability in model hair follicle systems. Hair follicle proliferation model data also notes extended anagen-like signalling and reduced keratinocyte apoptosis markers in organ-cultured follicle models. This dual GHK/biotin mechanism differentiates Biotinoyl Tripeptide-1 from single-mechanism hair actives — the peptide delivers both extracellular anchoring support and intracellular keratin synthesis co-factor within a single molecule.
Biotinoyl Tripeptide-1 builds targeted hair and lash density systems with Acetyl Tetrapeptide-3 (Folixyl) — targeting the 5α-reductase and hair loss cascade — and Myristoyl Hexapeptide-16 for stem cell niche lipopeptide support. For comprehensive lash concentrate positioning, combine with Myristoyl Pentapeptide-17 for lash shaft keratin synthesis alongside Biotinoyl Tripeptide-1’s follicle anchoring mechanism. Contact info@tcspeptides.com with formulation specification.
> Application Concepts
Lash Serum, Brow Concentrate and Scalp Density Architecture
Biotinoyl Tripeptide-1 at 0.001–0.1% dissolves directly in deionised water and is added at cool-down below 40°C, making it a clean addition to water-based lash serums, brow concentrates and scalp treatment formulations. At pH 5.0–6.5, it is compatible with standard aqueous serum bases containing carbomer, hyaluronic acid and panthenol. For a dual-mechanism lash concentrate, combine Biotinoyl Tripeptide-1 (follicle anchoring / fibronectin receptor) with Myristoyl Pentapeptide-17 (keratin synthesis stimulation in lash shaft) in a shared aqueous/PG base — Biotinoyl Tripeptide-1 dissolves in water; Myristoyl Pentapeptide-17 requires PG pre-dispersion. Add both peptide solutions sequentially at cool-down for a two-mechanism lash serum concentrate.
Biotin + GHK Dual Mechanism — Follicle Anchoring and Keratin Synthesis Co-Factor in One Molecule
Hair density and retention involves two independent targets: follicle-to-dermis anchoring (fibronectin/laminin ECM adhesion at the dermal papilla — the GHK component) and hair shaft keratin synthesis capacity (biotin-dependent carboxylase enzymes in proliferating matrix keratinocytes — the biotin component). Isolated biotin supplementation addresses the keratin synthesis co-factor pathway but not the follicle ECM anchoring deficit. Isolated GHK targets fibronectin receptor signalling but not biotin-dependent keratin production. Biotinoyl Tripeptide-1 addresses both pathways in a single ingredient — a structural advantage over dual-ingredient combinations where biotin and GHK are added separately without molecular linkage. For follicular AGE protection and miniaturisation prevention, additionally incorporate Acetyl Tetrapeptide-3 (Folixyl) targeting the 5α-R/DHT pathway.
Professional Lash, Clinical Brow and Scalp Density Serum Format Applications
Biotinoyl Tripeptide-1 addresses three premium hair-category market segments: professional lash extension-compatible serums (follicle anchoring to extend lash retention duration), clinical brow-density concentrates (follicle proliferation and anchoring for thinning brow rehabilitation), and scalp hair-density serums (GHK fibronectin receptor activation at the dermal papilla). All three formats benefit from the peptide’s clean water solubility, allowing serum-weight clear product architectures without silicone carriers or heavy emollients. For premium scalp positioning, evaluate alongside caffeine (2–4%) for vasodilation and follicle circulation support and Acetyl Tetrapeptide-3 for 5α-reductase pathway coverage, creating a three-mechanism density serum with independent molecular targets.
> Handling & Formulation Notes
Dissolve Biotinoyl Tripeptide-1 directly in deionised water or the aqueous phase at target concentrations (0.001–0.1%) without co-solvent, heating or pre-dispersion step. Add the aqueous solution to the formulation at cool-down below 40°C. No PG pre-dispersion is required at evaluation concentrations. For concentrations above 0.5%, prepare a 1% aqueous stock solution and add the calculated aliquot at cool-down.
Confirm stability of Biotinoyl Tripeptide-1 in the complete hair serum formulation at 40°C/75% RH for 12 weeks before scale-up. Monitor biotin amide bond and GHK peptide integrity by HPLC when formulating at pH outside 4.5–7.0 or in the presence of strong oxidising agents. Follow SDS and batch COA for storage and handling; confirm shelf life for the specific batch.
> Supply & Documentation
Biotinoyl Tripeptide-1 (CAS 299157-54-3) is available from TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic raw material projects. Standard documentation: COA, TDS, SDS. Sample and bulk quantities are discussed on a per-project basis; contact info@tcspeptides.com to initiate a sourcing review.
TCS NEXUS S.L. supports procurement and formulation teams in Germany, France, Italy, Spain, the United Kingdom, the Netherlands, Belgium, Poland, Sweden and Portugal, as well as international buyers in the United States, Canada, Japan, South Korea, Australia and New Zealand.
> Packaging & Storage
Biotinoyl Tripeptide-1 is supplied in sealed aluminium foil bags or bulk containers. Minimum quantities and packaging configurations are confirmed per order. Store below 25°C in a cool, dry location, away from moisture and direct light. Follow batch COA and SDS for specific storage and handling conditions.
> FAQ
What is Biotinoyl Tripeptide-1?
Biotinoyl Tripeptide-1 (CAS 299157-54-3) is a cosmetic raw material supplied by TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic formulators. In cosmetic literature, Biotinoyl Tripeptide-1 is positioned as a scalp and hair-follicle anchoring active — evaluated in anti-shedding leave-on serums targeting the follicular basement membrane proteins (laminin-5, collagen IV) that anchor the hair bulb within the dermal papilla. TCS NEXUS S.L. supplies Biotinoyl Tripeptide-1 as a B2B active for scalp and hair-care formulators.
What is the CAS number for Biotinoyl Tripeptide-1?
The CAS number for Biotinoyl Tripeptide-1 is 299157-54-3. The INCI name for cosmetic regulatory documentation and CPNP notification is Biotinoyl Tripeptide-1; confirm CAS 299157-54-3 and INCI identity against the batch COA when building product files.
How does Biotinoyl Tripeptide-1 (Biotin-GHK) support hair anchoring — what is the mechanism?
Biotinoyl Tripeptide-1 (Biotin-GHK) links the matrix-signalling GHK tripeptide (Gly-His-Lys) to biotin. The GHK part signals through the fibronectin receptor to support the anchoring proteins around the hair follicle, while the biotin moiety supports keratin-related metabolism — together positioned for the appearance of stronger anchoring and density in lash, brow and scalp products. It is a cosmetic conditioning peptide for hair and lash appearance, not a medicine.
What use level of Biotinoyl Tripeptide-1 is typical, and how should it be evaluated?
Treat the use level as project-specific and establish it conservatively for your base and claim rather than to a fixed figure; it is often supplied as a diluted trade solution, so confirm your dosing basis. We provide the pure material with COA — contact info@tcspeptides.com to align documentation.
Which actives pair with Biotinoyl Tripeptide-1 in a lash or scalp system?
For hair-appearance systems Biotinoyl Tripeptide-1 (follicle anchoring) pairs with Acetyl Tetrapeptide-3 (Folixyl), which targets the 5α-reductase pathway, and in lash concepts with keratin- and stem-cell peptides such as Myristoyl Pentapeptide-17 and Myristoyl Hexapeptide-16, plus panthenol and caffeine. It is water-soluble; confirm combined stability and pH in the finished base.
What makes Biotinoyl Tripeptide-1 distinct among hair peptides?
Its distinctive feature is the biotin–GHK conjugate design: it combines GHK matrix-anchoring signalling with biotin, targeting the follicle-anchoring appearance rather than keratin synthesis (Myristoyl Pentapeptide-17) or the hormonal 5α-reductase pathway (Acetyl Tetrapeptide-3). That makes it a complementary partner in multi-peptide hair and lash systems. Biotinoyl Tripeptide-1 is freely water-soluble.
What addition temperature is recommended for Biotinoyl Tripeptide-1 during manufacturing?
Introduce Biotinoyl Tripeptide-1 during the cool-down phase at temperatures below 40°C; avoid prolonged exposure above this threshold. Standard aqueous or emulsion cool-down protocols are fully compatible with this addition requirement. Confirm thermal stability in the specific manufacturing process with accelerated stability data before committing to production scale.
What pH range keeps Biotinoyl Tripeptide-1 stable in a finished formula?
Biotinoyl Tripeptide-1 is most stable at roughly pH 4.5–7.0, matching lash serums, scalp tonics and light emulsions; confirm the finished-product pH after all actives are incorporated and evaluate combined stability with any low-pH co-actives.
Is Biotinoyl Tripeptide-1 compatible with other actives in multi-active cosmetic systems?
Biotinoyl Tripeptide-1 is compatible with standard cosmetic actives at pH 4.5–7.0 in aqueous or emulsion systems. Evaluate combined system pH drift, clarity and assay retention; confirm preservative compatibility. Run accelerated stability testing at 40°C/75% RH before scale-up.
Is Biotinoyl Tripeptide-1 (INCI) compliant with EU Cosmetics Regulation 1223/2009?
Biotinoyl Tripeptide-1 is not listed as a restricted, prohibited or regulated substance under EU Cosmetics Regulation 1223/2009 Annexes II–VI. The finished product manufacturer is responsible for the CPSR and CPNP notification. Use CAS 299157-54-3 and INCI name Biotinoyl Tripeptide-1 consistently in all regulatory documentation; confirm purity and identity against the batch COA.
How should Biotinoyl Tripeptide-1 be stored?
Store Biotinoyl Tripeptide-1 sealed below 25°C in a cool, dry location protected from direct light and moisture. Once opened, reseal the container immediately. Follow specific storage and handling conditions stated in the SDS and batch COA. Confirm shelf life for the specific batch before incorporating into production formulations.
What documentation does TCS NEXUS S.L. provide for Biotinoyl Tripeptide-1?
TCS NEXUS S.L. provides COA (confirming CAS 299157-54-3 and HPLC purity ≥98% (HPLC)), TDS (Technical Data Sheet) and SDS (Safety Data Sheet) for Biotinoyl Tripeptide-1. Additional documentation such as specification sheets and INCI confirmation letters is available on a per-project basis. Contact info@tcspeptides.com to initiate a documentation and sourcing discussion.
What MOQ and sample options are available for Biotinoyl Tripeptide-1?
Sample and bulk quantities for Biotinoyl Tripeptide-1 are confirmed on a per-project basis through TCS NEXUS S.L. Minimum order quantities depend on current stock, packaging configuration and project scope. R&D teams are encouraged to request samples for stability and concentration-range evaluation before bulk planning. Contact info@tcspeptides.com with project specification to begin a sourcing review.
> Technical Support and Samples
Request COA documents, sample availability, or support for custom synthesis.