Pentapeptide-3 (KTTKS) – CAS 135679-88-8
Pentapeptide-3 is the water-soluble five-amino-acid sequence Lys-Thr-Thr-Lys-Ser (KTTKS), a fragment of type I procollagen that serves as one of the foundational collagen-signalling peptides in cosmetic science. With a molecular weight of approximately 550 Da and CAS number 135679-88-8, this peptide represents the precursor concept behind Matrixyl (Palmitoyl Pentapeptide-4), but without the palmitoyl lipid chain. The absence of the fatty acid tail gives Pentapeptide-3 full water solubility, opening formulation possibilities that lipopeptide variants cannot address.
Mechanism of Action
The KTTKS sequence was originally identified as a cleavage fragment released during the processing of type I procollagen into mature collagen fibrils. When this fragment is present in the extracellular space, fibroblasts interpret it as a signal that matrix turnover is occurring and respond by upregulating synthesis of collagens I and III, fibronectin, and elastin. This positive feedback mechanism makes KTTKS a classical matrikine: a peptide fragment of structural proteins that drives its own replacement. Pentapeptide-3 delivers this signal without lipid modification, relying on aqueous diffusion rather than membrane partitioning for dermal delivery.
Pentapeptide-3 vs. Palmitoyl Pentapeptide-4
The palmitoylated version (Matrixyl) adds a C16 fatty acid to the N-terminus of KTTKS, improving skin penetration through lipid bilayer interaction. Pentapeptide-3 lacks this modification and is therefore fully water-soluble, making it the preferred choice for aqueous-only systems such as toners, mists, hydrogel masks, and water-gel serums where incorporating a lipopeptide would compromise product clarity or texture. In emulsion systems, formulators often combine both forms: the palmitoylated version in the oil phase and Pentapeptide-3 in the aqueous phase for dual-route delivery.
Formulation Guidelines
Incorporate Pentapeptide-3 at 50-200 ppm in the aqueous phase. The peptide dissolves readily in water and glycol-water blends. Optimal pH range is 5.0-7.0. Stable at processing temperatures up to 40 degrees C. Compatible with hyaluronic acid, niacinamide, panthenol, and most non-ionic and amphoteric surfactants. Avoid combining with strong proteolytic enzymes (papain, bromelain) in leave-on products, as these may cleave the peptide bond during shelf life. For enhanced penetration in aqueous formulations, consider pairing with penetration enhancers like pentylene glycol or low-molecular-weight hyaluronic acid.
Applications
- Water-gel serums and clear aqueous treatments requiring no oil phase
- Hydrogel sheet masks and bio-cellulose masks for intensive firming
- Facial mists and toners delivering collagen-signal peptides throughout the day
- Dual-peptide emulsions combining Pentapeptide-3 (aqueous) with Matrixyl (oil phase)
- Body smoothing sprays targeting crepey skin on arms and legs
Quality and Supply from TCS NEXUS S.L.
TCS NEXUS S.L. supplies Pentapeptide-3 from Valencia, Spain with HPLC purity of 98% minimum. Each lot includes a certificate of analysis covering mass spectrometry identity, HPLC purity, peptide content, amino acid composition, and microbial limits. We maintain inventory for fast European dispatch and support orders from research-scale milligrams to production-scale kilograms. Regulatory documentation for EU Cosmetics Regulation (EC) No 1223/2009 compliance is available on request.
Frequently Asked Questions
Is Pentapeptide-3 the same as Matrixyl?
No. Matrixyl is Palmitoyl Pentapeptide-4, which adds a C16 fatty acid chain to the KTTKS sequence. Pentapeptide-3 is the unmodified, water-soluble version of the same collagen fragment without lipid conjugation.
What concentration of Pentapeptide-3 is recommended?
Use 50-200 ppm in the finished product. Most commercial formulations target 100 ppm for measurable collagen-signalling activity balanced against raw material cost.
Can Pentapeptide-3 be used in clear aqueous formulations?
Yes. Its full water solubility makes it ideal for toners, mists, hydrogel masks, and water-gel serums where lipopeptide variants would cause turbidity or phase separation.
Technical Support and Samples
Request COA documents, sample availability, or support for custom synthesis.
Frequently Asked Questions
Do you provide COA documents for Pentapeptide-3 Supplier Europe?
Yes. Each lot can be supplied with a Certificate of Analysis, HPLC purity data, and relevant quality-control results for technical review.
Are samples available for Pentapeptide-3 Supplier Europe?
Yes. Sample availability can be confirmed for formulation screening and internal evaluation before a commercial order is placed.
What is the delivery lead time for Pentapeptide-3 Supplier Europe?
For stocked material in Valencia, EU delivery is typically 5 to 7 business days. Final timing depends on destination and requested volume.
Do you also support custom synthesis and technical inquiries?
Yes. The team can help with custom synthesis, raw-material selection, technical documentation, and supply planning.