Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) (CAS 823202-99-9) is supplied by TCS NEXUS S.L. (Valencia, Spain) for European cosmetic formulators, brand R&D teams and B2B buyers reviewing cosmetic peptide raw material applications. A freely water-soluble peptide, it dissolves directly in aqueous concentrates without co-solvents at pH 4.5–7.0, with cool-down addition below 45°C recommended in standard serum and leave-on manufacturing protocols.
> Technical Specifications
| Product Name | Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) |
|---|---|
| Molecular Structure |  |
| CAS Number | 823202-99-9 |
| Alias / Common Name | Dipeptide Diaminobutyroyl Benzylamide Diacetate; Syn-Ake; Waglerin-1 synthetic analog |
| Molecular Formula | C23H37N5O7 |
| Molecular Weight | 495.6 g/mol |
| INCI Name | Dipeptide Diaminobutyroyl Benzylamide Diacetate |
| Peptide Sequence | Ac-β-Ala-diaminobutyroyl benzylamide diacetate — Waglerin-1 biomimetic (nAChR postsynaptic) |
| Functional Class | Synthetic dipeptide; postsynaptic nAChR modulator; expression-line active |
| Purity / Assay | ≥98% (HPLC); confirm by batch COA |
| Appearance | White to off-white powder |
| Solubility / Handling | Freely water-soluble; dissolve in deionised water or aqueous phase before incorporation; no co-solvent required |
| pH Stability | Optimal stability at pH 4.5–7.0; confirm in target base after all actives incorporated |
| Storage | Store sealed in cool, dry conditions protected from direct light and moisture; follow batch COA and SDS |
> Mechanism & Positioning
Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake, CAS 823202-99-9) is a synthetic structural analog of Waglerin-1, a peptide from the venom of the temple viper (Tropidolaemus wagleri), designed to modulate postsynaptic nicotinic acetylcholine receptors (nAChR) at the neuromuscular junction. Published Syn-Ake literature discusses that the peptide acts at the postsynaptic nAChR — specifically the acetylcholine binding site on the muscle-type nAChR (α1/β1/δ/ε subunit configuration) — interfering with acetylcholine-receptor binding and attenuating postsynaptic depolarisation. Cell-based neuromuscular studies document reduced muscle contraction signalling in in vitro neuromuscular model assays at evaluated peptide concentrations, consistent with attenuated postsynaptic nAChR activation. Postsynaptic nAChR model data also notes that the diaminobutyroyl benzylamide structure provides competitive receptor interaction at the acetylcholine binding site without permanent receptor blockade — consistent with the cosmetic raw material profile.
Based on available in vitro data, Dipeptide Diaminobutyroyl Benzylamide Diacetate is positioned for expression-line serum, anti-wrinkle concentrate and periorbital formulation concepts where postsynaptic nAChR modulation is the primary mechanistic direction. The postsynaptic nAChR mechanism differentiates Syn-Ake from presynaptic SNARE pathway actives such as Argireline (Acetyl Hexapeptide-8) and from GABAergic presynaptic Ca²⁺ modulators such as Leuphasyl (Pentapeptide-18), enabling a three-mechanism neuromuscular signal attenuation system covering presynaptic SNARE inhibition, GABAergic Ca²⁺ modulation and postsynaptic nAChR modulation across the neuromuscular axis.
> Application Concepts
Expression Line Serum Architecture
Dipeptide Diaminobutyroyl Benzylamide Diacetate is freely water-soluble and dissolves directly in glycerin–water concentrates, hyaluronic acid solutions and buffered aqueous serum bases at evaluation concentrations of 0.001–0.01% (w/w) without co-solvent or glycol pre-mix. Introduce during the cool-down phase below 45°C; standard serum manufacturing cool-down protocols are fully compatible. The water-soluble profile allows clean addition to transparent serum formats, layering concentrates and pump-format products where clarity and simple INCI declaration are formulation priorities. For multi-mechanism neuromuscular positioning, Syn-Ake (postsynaptic nAChR) combines cleanly with Argireline (SNARE complex) and Leuphasyl (GABAergic Ca²⁺) — all three are water-soluble, stable at pH 4.5–7.0 and added during cool-down below 45°C in a single manufacturing step. Confirm pH (4.5–7.0) and peptide assay retention via accelerated stability testing at 40°C/75% RH before scale-up.
Three-Mechanism Neuromuscular Relaxation Systems
Dipeptide Diaminobutyroyl Benzylamide Diacetate (postsynaptic nAChR) completes a three-mechanism neuromuscular system with Argireline (Acetyl Hexapeptide-8) — SNARE complex / SNAP-25 N-terminal biomimetic targeting vesicle docking; and Leuphasyl (Pentapeptide-18) — GABAergic pathway / enkephalin receptor agonism reducing presynaptic Ca²⁺ influx. The three mechanisms target independent steps in the neuromuscular signalling cascade: presynaptic SNARE inhibition (Argireline/SNAP-8), presynaptic Ca²⁺ attenuation (Leuphasyl) and postsynaptic nAChR modulation (Syn-Ake). All three are freely water-soluble, stable at pH 4.5–7.0 and can be combined in a single aqueous serum base at their respective evaluation concentrations (0.001–0.01% each) without auxiliary solvents or separate phase incorporation. Confirm combined peptide assay retention, pH and preservative compatibility before scale-up.
Periorbital and Targeted Relaxation Formulations
The postsynaptic nAChR mechanism makes Dipeptide Diaminobutyroyl Benzylamide Diacetate particularly relevant for periorbital and forehead treatments where expression lines from repeated facial muscle contraction are the formulation target. At 0.001–0.01% (w/w), Syn-Ake integrates into lightweight eye-contour gels, cream-gel eye treatments and hydrogel patches without auxiliary solvents — keeping periorbital formulation systems clean and well-tolerated. Evaluate compatibility with niacinamide (2–5%), caffeine and hyaluronic acid at pH 5.0–6.5; confirm pH alignment across all actives. For comprehensive periorbital neuromuscular positioning, Syn-Ake (postsynaptic) may be evaluated alongside Argireline (presynaptic SNARE) for complementary pre- and post-synaptic pathway coverage in a single periorbital concentrate. Confirm combined stability and assay retention before scale-up.
> Handling & Formulation Notes
Dissolve Dipeptide Diaminobutyroyl Benzylamide Diacetate in deionised water or the aqueous phase at ambient temperature; the peptide is freely water-soluble and does not require co-solvent, glycol pre-mix or surfactant dispersion. Introduce the aqueous solution during the cool-down phase at temperatures below 45°C. Maintain formulation pH within 4.5–7.0 for optimal stability; confirm pH after all actives and buffering agents are incorporated into the finished batch.
Evaluate compatibility with EDTA-containing preservative systems and other chelating agents at typical cosmetic concentrations. Confirm colour, clarity and peptide assay retention via accelerated stability testing at 40°C/75% RH across the intended shelf-life period before scale-up to production batches.
> Supply & Documentation
Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) (CAS 823202-99-9) is available from TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic raw material projects. Standard documentation: COA, TDS, SDS. Sample and bulk quantities are discussed on a per-project basis; contact info@tcspeptides.com to initiate a sourcing review.
TCS NEXUS S.L. supports procurement and formulation teams in Germany, France, Italy, Spain, the United Kingdom, the Netherlands, Belgium, Poland, Sweden and Portugal, as well as international buyers in the United States, Canada, Japan, South Korea, Australia and New Zealand.
> Packaging & Storage
Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) is supplied in sealed aluminium foil bags or bulk containers. Minimum quantities and packaging configurations are confirmed per order. Store below 25°C in a cool, dry location, away from moisture and direct light. Follow batch COA and SDS for specific storage and handling conditions.
> FAQ
What is Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake)?
Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) (CAS 823202-99-9) is a cosmetic raw material supplied by TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic formulators. Cosmetically, it is evaluated for its ability to reduce the appearance of expression lines via modulation of muscular nicotinic acetylcholine receptor (nAChR) signalling at the neuromuscular junction. TCS NEXUS S.L. supplies Syn-Ake as a pure peptide diacetate salt for incorporation into anti-ageing serums, eye creams and expression-line-targeting leave-on systems.
What is the CAS number for Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake)?
The CAS number for Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) is 823202-99-9. The INCI name for cosmetic regulatory documentation and CPNP notification is Dipeptide Diaminobutyroyl Benzylamide Diacetate; confirm CAS 823202-99-9 and INCI identity against the batch COA when building product files.
How does Syn-Ake block contraction — what is the postsynaptic nAChR mechanism?
Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) is a synthetic mimic of Waglerin-1, a peptide from temple-viper venom. It acts at the postsynaptic membrane as a reversible antagonist of the muscle-type nicotinic acetylcholine receptor (nAChR): by occupying the receptor it lowers the ion flux that triggers muscle-fibre contraction. This postsynaptic site is what distinguishes Syn-Ake from presynaptic SNARE peptides, positioning it for expression-line and mimic-wrinkle claim directions.
What use level of Syn-Ake is typical, and does trade-solution vs pure-peptide basis matter?
Because the effective level shifts with formulation, claim and protocol, evaluate it conservatively and confirm it in your own trials rather than assuming a fixed figure. Note whether you are dosing the pure Dipeptide Diaminobutyroyl Benzylamide Diacetate raw material or a diluted trade solution, since market references often quote the latter. We supply the pure peptide with COA so you can dose on a pure-peptide basis; contact info@tcspeptides.com to align documentation with your target.
Which peptides combine with Syn-Ake for a multi-pathway expression-line system?
Because Syn-Ake acts postsynaptically at the nAChR, it pairs with peptides that target other steps of the neuromuscular axis: presynaptic SNARE inhibitors such as Argireline (Acetyl Hexapeptide-8) and SNAP-8 (Acetyl Octapeptide-3), and the GABAergic modulator Leuphasyl (Pentapeptide-18). Addressing the contraction signal from both the pre- and postsynaptic side is the rationale for these combinations. All are water-soluble; confirm combined assay retention and pH in the finished base.
What is Waglerin-1, and how does Syn-Ake relate to it?
Waglerin-1 is a peptide found in the venom of the temple viper (Tropidolaemus wagleri) that binds the muscle nicotinic acetylcholine receptor. Syn-Ake — INCI Dipeptide Diaminobutyroyl Benzylamide Diacetate — is a short, freely water-soluble synthetic analogue designed to reproduce that receptor-binding activity in a cosmetic-grade raw material, with no animal-derived venom involved. Being water-soluble, it dissolves directly in the aqueous phase.
How should Syn-Ake be incorporated during manufacturing?
Dissolve Syn-Ake in deionised water or the aqueous phase — no co-solvent is required — and introduce it during the cool-down phase below 45°C, since prolonged heat above this point risks peptide degradation. Standard aqueous serum and light-emulsion cool-down protocols suit it well; verify assay retention with accelerated stability testing before scale-up.
What pH range keeps Syn-Ake stable in a finished formula?
Syn-Ake holds up best across pH 4.5–7.0. As its target is a membrane receptor rather than a pH-sensitive enzyme, formulation pH mostly governs peptide stability rather than the mode of action — but keep it out of strongly acidic bases below pH 4.5 and away from high-dose ascorbic-acid serums, and confirm the finished-product pH once all actives are incorporated.
Is Syn-Ake suitable for periorbital and forehead expression-line treatments?
Yes — as a postsynaptic nAChR modulator, Syn-Ake fits periorbital and forehead expression-line treatments and combines naturally with presynaptic peptides in the same product. Its water solubility keeps eye-contour gels, cream-gels and hydrogel patches free of auxiliary solvents; check compatibility with eye-area actives such as niacinamide and caffeine and align pH across the finished system.
Is Dipeptide Diaminobutyroyl Benzylamide Diacetate (INCI) compliant with EU Cosmetics Regulation 1223/2009?
Dipeptide Diaminobutyroyl Benzylamide Diacetate is not listed as a restricted, prohibited or regulated substance under EU Cosmetics Regulation 1223/2009 Annexes II–VI. The finished product manufacturer is responsible for the CPSR and CPNP notification. Use CAS 823202-99-9 and INCI name Dipeptide Diaminobutyroyl Benzylamide Diacetate consistently in all regulatory documentation; confirm purity and identity against the batch COA.
How should Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) be stored?
Store Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) sealed below 25°C in a cool, dry location protected from direct light and moisture. Once opened, reseal the container immediately. Follow specific storage and handling conditions stated in the SDS and batch COA. Confirm shelf life for the specific batch before incorporating into production formulations.
What documentation does TCS NEXUS S.L. provide for Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake)?
TCS NEXUS S.L. provides COA (confirming CAS 823202-99-9 and HPLC purity ≥98% (HPLC)), TDS (Technical Data Sheet) and SDS (Safety Data Sheet) for Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake). Additional documentation such as specification sheets and INCI confirmation letters is available on a per-project basis. Contact info@tcspeptides.com to initiate a documentation and sourcing discussion.
What MOQ and sample options are available for Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake)?
Sample and bulk quantities for Dipeptide Diaminobutyroyl Benzylamide Diacetate (Syn-Ake) are confirmed on a per-project basis through TCS NEXUS S.L. Minimum order quantities depend on current stock, packaging configuration and project scope. R&D teams are encouraged to request samples for stability and concentration-range evaluation before bulk planning. Contact info@tcspeptides.com with project specification to begin a sourcing review.
> Technical Support and Samples
Request COA documents, sample availability, or support for custom synthesis.