Acetyl Tetrapeptide-5: Periorbital Peptide for Puffiness and Dark Circles
Acetyl Tetrapeptide-5 (INCI: Acetyl Tetrapeptide-5, CAS 820959-17-9) is a four-amino-acid acetylated peptide with a molecular weight of 492.53 g/mol. Its sequence – Ac-beta-Ala-His-Ser-His – was designed to address the two primary causes of under-eye concerns: fluid accumulation (puffiness) and vascular-related discoloration (dark circles). This peptide belongs to the Eyeseryl-type category and has become a staple active in professional eye-care formulations worldwide.
Mechanism of Action: Anti-Edema and Anti-Glycation
The periorbital area is uniquely susceptible to fluid retention due to its thin dermis and dense capillary network. Acetyl Tetrapeptide-5 works through two complementary pathways. First, it inhibits angiotensin-converting enzyme (ACE), which plays a direct role in regulating vascular permeability. By reducing ACE activity, the peptide limits plasma leakage from capillaries into the surrounding tissue, directly addressing the edema that manifests as under-eye bags. Second, it reduces advanced glycation end-product (AGE) formation in the extracellular matrix. Glycation cross-links collagen fibers, reducing tissue elasticity and trapping fluids – Acetyl Tetrapeptide-5 interrupts this process, helping maintain dermal flexibility around the eye contour.
Clinical Efficacy Data
In a controlled 60-day clinical study with 20 volunteers, a formulation containing 0.005% Acetyl Tetrapeptide-5 produced measurable improvements in two key parameters. Under-eye puffiness decreased by 32% as assessed by standardized photography and profilometric analysis. Dark circle severity scores improved by 22% based on colorimetric measurements of the periorbital area. These results were statistically significant compared to the placebo group, and participants reported noticeable improvement starting from week three of twice-daily application.
Formulation Guidelines
Acetyl Tetrapeptide-5 is water-soluble and stable across a pH range of 5.0 to 7.0. Recommended incorporation level ranges from 100 to 1000 ppm in finished products. Processing temperature must stay below 45 degrees Celsius – incorporate during the cool-down phase of any emulsion process. The peptide is compatible with standard cosmetic emollients, humectants, and preservative systems. For enhanced periorbital formulations, consider combining with Dipeptide-2 (lymphatic drainage support) or Hexapeptide-9 (collagen stimulation around the eye area).
Target Applications
- Eye creams and eye serums targeting puffiness and dark circles
- Under-eye hydrogel patches with sustained peptide release
- Anti-fatigue eye contour gels for morning application
- Multi-peptide eye treatments combining Dipeptide-2 and Hexapeptide-9
- Premium anti-aging regimens with dedicated eye-zone actives
Technical Specifications
| Parameter | Value |
|---|---|
| INCI Name | Acetyl Tetrapeptide-5 |
| CAS Number | 820959-17-9 |
| Molecular Weight | 492.53 g/mol |
| Purity (HPLC) | ≥98% |
| Solubility | Water soluble |
| Recommended pH | 5.0 – 7.0 |
| Use Level | 100 – 1000 ppm |
| Storage | Below 45 C, protect from light |
Sourcing from TCS NEXUS S.L.
TCS NEXUS S.L. in Valencia, Spain, produces Acetyl Tetrapeptide-5s. Each lot includes a Certificate of Analysis with HPLC purity data, mass spectrometry verification, and peptide content determination. Evaluation orders start at 1 g, with production quantities from 100 g. EU delivery is completed within 5 to 7 business days.
Frequently Asked Questions
What makes Acetyl Tetrapeptide-5 effective for under-eye puffiness?
The peptide inhibits angiotensin-converting enzyme, which regulates vascular permeability in the periorbital capillary network. By reducing ACE activity, it limits fluid leakage from blood vessels into surrounding tissue. Clinical data shows a 32% reduction in puffiness over 60 days at just 0.005% concentration, confirming its potency at low use levels.
Can Acetyl Tetrapeptide-5 address both puffiness and dark circles?
Yes. The dual mechanism – ACE inhibition for edema reduction and anti-glycation for improved tissue elasticity – addresses both concerns simultaneously. The same clinical study demonstrated 32% puffiness reduction alongside 22% improvement in dark circle scores, making it a comprehensive periorbital active.
What is the recommended combination for a professional eye serum?
For a multi-pathway eye serum, formulators typically combine Acetyl Tetrapeptide-5 (50-200 ppm) with Dipeptide-2 for lymphatic drainage support and Hexapeptide-9 for periorbital collagen stimulation. This three-peptide approach targets fluid retention, vascular discoloration, and fine lines around the eye contour.
Technical Support and Samples
Request COA documents, sample availability, or support for custom synthesis.
Frequently Asked Questions
Do you provide COA documents for Acetyl Tetrapeptide-5 Supplier Europe?
Yes. Each lot can be supplied with a Certificate of Analysis, HPLC purity data, and relevant quality-control results for technical review.
Are samples available for Acetyl Tetrapeptide-5 Supplier Europe?
Yes. Sample availability can be confirmed for formulation screening and internal evaluation before a commercial order is placed.
What is the delivery lead time for Acetyl Tetrapeptide-5 Supplier Europe?
For stocked material in Valencia, EU delivery is typically 5 to 7 business days. Final timing depends on destination and requested volume.
Do you also support custom synthesis and technical inquiries?
Yes. The team can help with custom synthesis, raw-material selection, technical documentation, and supply planning.