NAD+ (Nicotinamide Adenine Dinucleotide) (CAS 53-84-9) is supplied by TCS NEXUS S.L. (Valencia, Spain) for European cosmetic formulators, brand R&D teams and B2B buyers reviewing cosmetic active ingredient and coenzyme raw material applications. A freely water-soluble coenzyme, it dissolves directly in aqueous concentrates without co-solvents at pH 4.5–6.5, with cool-down addition below 40°C recommended in standard serum and leave-on manufacturing protocols.
> Technical Specifications
| Product Name | NAD+ (Nicotinamide Adenine Dinucleotide) |
|---|---|
| Molecular Structure |  |
| CAS Number | 53-84-9 |
| Alias / Common Name | NAD+; Nicotinamide Adenine Dinucleotide; NAD; coenzyme I |
| Molecular Formula | C21H27N7O14P2 |
| Molecular Weight | 663.4 g/mol |
| INCI Name | Nicotinamide Adenine Dinucleotide |
| Peptide Sequence | Nicotinamide + adenine dinucleotide — NAD+ oxidised form (sirtuin substrate coenzyme) |
| Functional Class | Naturally occurring coenzyme; sirtuin (SIRT1–7) activator substrate; PARP cofactor; cellular NAD+ metabolism active; skin longevity and mitochondrial energy support ingredient |
| Purity / Assay | ≥95% (HPLC); confirm by batch COA |
| Appearance | White to off-white crystalline powder |
| Solubility / Handling | Freely water-soluble at evaluation concentrations (0.1–2.0%); dissolves directly in deionised water or the aqueous phase without co-solvent. Add at cool-down below 40°C. |
| pH Stability | Optimal stability at pH 4.5–6.5 in aqueous systems. Evaluate stability carefully at pH above 7.0 — NAD+ is susceptible to alkaline hydrolysis. Confirm formulation pH with buffer control. |
| Storage | Store sealed below -20°C (frozen) or below 4°C (refrigerated) for long-term stability. Short-term storage below 25°C with moisture and light protection acceptable. Follow SDS and batch COA conditions. |
> Mechanism & Positioning
NAD+ (Nicotinamide Adenine Dinucleotide) is the oxidised form of the essential cellular coenzyme, operating as a hydride-ion carrier in mitochondrial oxidative phosphorylation (Complex I, III) and as the obligate substrate for sirtuin deacylases (SIRT1–7) and PARP DNA-repair enzymes — all key mediators of skin cellular ageing. Published NAD+ biology literature discusses the central role of NAD+ depletion in the ageing skin phenotype: sirtuins (SIRT1, SIRT3, SIRT6) require NAD+ as a co-substrate to regulate p53 activity, NF-κB inflammatory signalling, mitochondrial biogenesis and telomere integrity — all of which decline as NAD+ pools decrease with chronological age and UV exposure. Cell-based keratinocyte and fibroblast studies document improved mitochondrial membrane potential, increased SIRT1 activity and attenuated ROS production following NAD+ or NAD+ precursor treatment. DNA-repair efficiency model data also notes PARP1-dependent base excision repair (BER) restoration in UV-damaged keratinocyte models, consistent with NAD+ availability as the rate-limiting factor in UV-induced DNA repair in the skin. Topical NAD+ supplementation at 0.1–2.0% aims to replenish the depleted epidermal and dermal NAD+ pools, supporting sirtuin-mediated epigenetic regulation and PARP-mediated genomic stability in ageing skin.
NAD+ builds metabolic longevity systems with NMN (Nicotinamide Mononucleotide) — a direct NAD+ biosynthesis precursor via the NRK/NMNAT pathway — for complementary intracellular NAD+ replenishment alongside direct topical NAD+ supplementation. For comprehensive longevity positioning, add Ergothioneine for TAUT-transporter-mediated antioxidant protection against mitochondrial ROS and Glutathione (GSH) for tripeptide redox balance support alongside NAD+ metabolism. Contact info@tcspeptides.com with formulation specification.
> Application Concepts
Longevity Serum and NAD+ Coenzyme Topical Architecture
NAD+ at 0.1–2.0% dissolves directly in deionised water and is added at cool-down below 40°C — formulate in a buffered aqueous serum at pH 5.0–6.0 to maintain NAD+ stability. Carbomer and hyaluronic acid-based gel serums at pH 5.5 provide an appropriate delivery vehicle for topical NAD+ application. Avoid formulating above pH 7.0 without careful stability screening — alkaline hydrolysis of the nicotinamide N-glycosidic bond degrades NAD+ rapidly at pH > 7.5. For a dual NAD+ precursor serum, combine NAD+ (0.5–1.0%) with NMN (0.5–1.0%) in a pH 5.5 buffered serum base — providing both the direct oxidised coenzyme and the biosynthesis pathway precursor in a single longevity concentrate.
NAD+ Sirtuin Pathway vs PARP DNA Repair Pathway — Two Longevity Mechanisms One Coenzyme
NAD+ depletion in ageing skin affects two mechanistically independent longevity pathways that share the coenzyme as the limiting substrate. Sirtuin pathway: SIRT1 (nucleus — NF-κB deacetylation → calming (NF-κB pathway), p53 deacetylation → senescence resistance), SIRT3 (mitochondria — Complex I activity → ROS control) and SIRT6 (telomere protection → genomic stability) all require NAD+ as the co-substrate for each deacylation reaction. PARP pathway: PARP1 and PARP2 execute base excision repair (BER) of UV-induced single-strand breaks using NAD+ as the ADP-ribose donor — consuming NAD+ during repair. As epidermal NAD+ decreases with age, both sirtuin-mediated epigenetic regulation and PARP-mediated DNA repair are simultaneously compromised — explaining the multi-dimensional age phenotype associated with NAD+ decline. Topical NAD+ with NMN targets both pathways by replenishing the shared coenzyme substrate.
Longevity Serum, DNA Repair and Mitochondrial Energy Skin Format Applications
NAD+ at 0.1–2.0% is positioned for three longevity and science-forward skin care segments: anti-ageing longevity serums (sirtuin/PARP mechanism — the “cellular energy” age care category), post-UV repair concentrates (PARP-dependent DNA repair restoration in UV-damaged skin), and mitochondrial skin energy serum concepts (Complex I support in chronologically aged skin). For a longevity ampoule concept, combine NAD+ (0.5%) with NMN (0.5%), Ergothioneine (0.5%) and niacinamide (4%) in a pH 5.5 serum base — the niacinamide provides an additional NAD+ precursor via the NAMPT salvage pathway, niacinamide inhibits melanosome transfer and Ergothioneine provides TAUT-mediated intracellular antioxidant support alongside NAD+ sirtuin activation.
> Handling & Formulation Notes
Dissolve NAD+ directly in deionised water or the aqueous phase at target concentrations (0.1–2.0%) without co-solvent or heating. Add to the formulation at cool-down below 40°C. Formulate in a buffered aqueous system at pH 5.0–6.0 (citrate or phosphate buffer at 10–50 mM) to prevent alkaline hydrolysis. Avoid exposure to metal ions (iron, copper) that can accelerate NAD+ oxidative degradation — use EDTA (0.1%) or phytic acid as chelant in the formulation.
NAD+ is less stable than peptide actives under typical cosmetic stability conditions. Confirm HPLC stability at 40°C/75% RH at 4-week intervals for 12 weeks before scale-up — monitor both NAD+ loss and AMP/ADPR degradation product formation. Consider packaging in nitrogen-flushed amber glass or single-use ampoule formats to minimise oxidative degradation during shelf life. Follow SDS and batch COA for storage and handling; confirm shelf life for the specific batch.
> Supply & Documentation
NAD+ (Nicotinamide Adenine Dinucleotide) (CAS 53-84-9) is available from TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic raw material projects. Standard documentation: COA, TDS, SDS. Sample and bulk quantities are discussed on a per-project basis; contact info@tcspeptides.com to initiate a sourcing review.
TCS NEXUS S.L. supports procurement and formulation teams in Germany, France, Italy, Spain, the United Kingdom, the Netherlands, Belgium, Poland, Sweden and Portugal, as well as international buyers in the United States, Canada, Japan, South Korea, Australia and New Zealand.
> Packaging & Storage
NAD+ (Nicotinamide Adenine Dinucleotide) is supplied in sealed aluminium foil bags or bulk containers. Minimum quantities and packaging configurations are confirmed per order. Store below 25°C in a cool, dry location, away from moisture and direct light. Follow batch COA and SDS for specific storage and handling conditions.
> FAQ
What is NAD+ (Nicotinamide Adenine Dinucleotide)?
NAD+ (Nicotinamide Adenine Dinucleotide) (CAS 53-84-9) is a cosmetic raw material supplied by TCS NEXUS S.L. (Valencia, Spain) for B2B cosmetic formulators. It is positioned for skin longevity serum, mitochondrial energy-care concentrate and NAD+-replenishment age-care formulations at 0.01–0.5%.
What is the CAS number for NAD+ (Nicotinamide Adenine Dinucleotide)?
The CAS number for NAD+ (Nicotinamide Adenine Dinucleotide) is 53-84-9. The INCI name for cosmetic regulatory documentation and CPNP notification is Nicotinamide Adenine Dinucleotide; confirm CAS 53-84-9 and INCI identity against the batch COA when building product files.
How does NAD+ drive skin-cell energy and repair — what is the coenzyme role?
NAD+ (Nicotinamide Adenine Dinucleotide), also called coenzyme I, is the central redox coenzyme of the cell. It shuttles electrons in mitochondrial energy metabolism (the NAD+/NADH couple) and is the direct substrate consumed by sirtuins (SIRT1–7) and by PARP enzymes during DNA repair. Skin NAD+ falls with age and UV exposure, so replenishing it is positioned for cellular-energy, resilience and age-care claim directions in cosmetic formulation.
What use level of NAD+ is typical, and how should it be evaluated?
Use level is formulation- and claim-specific, so assess it conservatively for your own base rather than adopting a fixed figure; the TDS references a low single-digit percent evaluation window as a starting point, adjusted to your base and claim. We supply pure NAD+ with COA so you can dose on a pure-material basis and monitor retention over shelf life; contact info@tcspeptides.com to align documentation with your target.
Which actives pair with NAD+ in an age-care or energy formula?
NAD+ combines with other NAD+-pathway and vitality actives: its precursor NMN (Nicotinamide Mononucleotide) and niacinamide (vitamin B3) further upstream, plus antioxidants such as vitamin E or sirtuin-support ingredients. Because NAD+ is more pH- and heat-sensitive than these, keep the shared system on the acidic side of pH 4.5–6.5, avoid alkaline co-actives, and confirm combined stability and NAD+ retention in the finished base.
How does NAD+ differ from its precursor NMN?
NAD+ is the finished coenzyme; NMN (Nicotinamide Mononucleotide) is the one-step precursor that cells convert into NAD+ via the NMNAT enzymes. Both are freely water-soluble, but NAD+ is the more delicate of the two to formulate — it is more prone to alkaline hydrolysis, so it favours a tighter, more acidic pH window. Formulators choosing between them often weigh direct-coenzyme delivery (NAD+) against the easier stability profile of the precursor (NMN). This raw material is NAD+ (CAS 53-84-9); TCS NEXUS S.L. also supplies NMN separately.
How should NAD+ be incorporated, and why keep temperatures low?
NAD+ is freely water-soluble — dissolve it directly in deionised water or the aqueous phase without a co-solvent, and add it late during cool-down below 40°C, since it degrades with heat. Cold-chain or refrigerated handling and low-headspace, moisture- and light-protected packaging help preserve NAD+ in the finished product; verify assay retention over the intended shelf life rather than assuming raw-material stability.
What pH range keeps NAD+ stable, and why avoid alkaline systems?
NAD+ is most stable at pH 4.5–6.5 and is susceptible to alkaline hydrolysis, so evaluate it carefully above pH 7.0 and use buffer control to hold the target window. This narrower, more acidic requirement — tighter than that of its precursor NMN — is the main formulation constraint. Confirm the finished-product pH and NAD+ retention after all actives and buffering agents are incorporated.
What cosmetic claim direction is NAD+ positioned for?
Its role as the cell’s energy and repair coenzyme positions NAD+ for skin-vitality, resilience and age-care claim directions in serums, essences and eye products, typically within antioxidant or NAD+-pathway systems. Keep claims within EU cosmetic boundaries — skin appearance and feel, not systemic or medicinal effects — and substantiate them with your own use and stability data.
Is Nicotinamide Adenine Dinucleotide (INCI) compliant with EU Cosmetics Regulation 1223/2009?
Nicotinamide Adenine Dinucleotide is not listed as a restricted, prohibited or regulated substance under EU Cosmetics Regulation 1223/2009 Annexes II–VI. The finished product manufacturer is responsible for the CPSR and CPNP notification. Use CAS 53-84-9 and INCI name Nicotinamide Adenine Dinucleotide consistently in all regulatory documentation; confirm purity and identity against the batch COA.
How should NAD+ (Nicotinamide Adenine Dinucleotide) be stored?
Store NAD+ (Nicotinamide Adenine Dinucleotide) sealed below 25°C in a cool, dry location protected from direct light and moisture. Once opened, reseal the container immediately. Follow specific storage and handling conditions stated in the SDS and batch COA. Confirm shelf life for the specific batch before incorporating into production formulations.
What documentation does TCS NEXUS S.L. provide for NAD+ (Nicotinamide Adenine Dinucleotide)?
TCS NEXUS S.L. provides COA (confirming CAS 53-84-9 and HPLC purity ≥95% (HPLC)), TDS (Technical Data Sheet) and SDS (Safety Data Sheet) for NAD+ (Nicotinamide Adenine Dinucleotide). Additional documentation such as specification sheets and INCI confirmation letters is available on a per-project basis. Contact info@tcspeptides.com to initiate a documentation and sourcing discussion.
What MOQ and sample options are available for NAD+ (Nicotinamide Adenine Dinucleotide)?
Sample and bulk quantities for NAD+ (Nicotinamide Adenine Dinucleotide) are confirmed on a per-project basis through TCS NEXUS S.L. Minimum order quantities depend on current stock, packaging configuration and project scope. R&D teams are encouraged to request samples for stability and concentration-range evaluation before bulk planning. Contact info@tcspeptides.com with project specification to begin a sourcing review.
> Technical Support and Samples
Request COA documents, sample availability, or support for custom synthesis.